Protein Synthesis during First Cleavage of Sea Urchin Embryos
نویسندگان
چکیده
منابع مشابه
Differential behavior of centrosomes in unequally dividing blastomeres during fourth cleavage of sea urchin embryos.
The fourth cleavage in sea urchin embryos is unequal and represents an initial step in cell differentiation: vegetal blastomeres divide to produce micromeres, which are precursors of the skeletogenic mesenchyme, and macromeres. The mitotic spindles of these unequally dividing cells are peripherally located and lie orthogonal to the vegetal pole plasma membrane; the aster of the micromere pole i...
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Studies employing colchicine binding, precipitation with vinblastine sulfate, and acrylamide gel electrophoresis confirm earlier proposals that Arbacia punctulata and Lytechinus pictus eggs and embryos contain a store of microtubule proteins. Treatment of 150,000 g supernatants from sea urchin homogenates with vinblastine sulfate precipitates about 5% of the total soluble protein, and 75% of th...
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Previous work using pulse-chase labelling of embryos from the sea-urchin Strongylocentrotus droebachiensis during ciliogenesis, regeneration or steady-state maintenance and elongation showed that a ciliary outer doublet microtubule-associated protein, originally termed component-20, was synthesized in a fixed or quantal amount. This suggested that the limited synthesis of component-20 might lim...
متن کاملFatty acylation of proteins during development of sea urchin embryos.
Developing embryos of the sea urchin, Strongylocentrotus purpuratus, incorporate [3H]palmitic acid into at least 20 proteins. The [3H]palmitic acid associated with these proteins is released by alkaline hydrolysis or by treatment with hydroxylamine but not by extensive extraction with chloroform:methanol, indicating that the fatty acids are covalently attached to protein. The finding that the f...
متن کاملA Cytochemical Study of the Sulfhydryl Groups of Sea Urchin Eggs during the First Cleavage
In the eggs of four species of echinoderms, Mespilia globulus, Pseudocentrotus depressus, Hemicentrotus pulcherrimus and Clypeaster japonicus, changes in the distribution of protein-bound SH groups from fertilization to the 2 cell stage have been studied cytochemically by use of a mercaptide-forming azo dye. In the eggs of these species, the color intensity in the cytoplasm increased upon ferti...
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ژورنال
عنوان ژورنال: Science
سال: 1966
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.154.3752.1055